Journal article
Anti-inflammatory properties of sirtuin 6 in human umbilical vein endothelial cells
M Lappas
Mediators of Inflammation | HINDAWI LTD | Published : 2012
DOI: 10.1155/2012/597514
Abstract
A prominent feature of inflammatory diseases is endothelial dysfunction. Factors associated with endothelial dysfunction include proinflammatory cytokines, adhesion molecules, and matrix degrading enzymes. At the transcriptional level, they are regulated by the histone deacetylase sirtuin (SIRT) 1 via its actions on the proinflammatory transcription factor nuclear factor- κ B (NF- κ B). The role of SIRT6, also a histone deacetylase, in regulating inflammation in endothelial cells is not known. The aim of this study was to determine the effect of SIRT6 knockdown on inflammatory markers in human umbilical vein endothelial cells (HUVECs) in the presence of lipopolysaccharide (LPS). LPS decrease..
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Grants
Awarded by National Health and Medical Research Council
Funding Acknowledgements
Gillian Barker (Department of Obstetrics and Gynaecology, University of Melbourne) is thanked for her help with the RNA extractions and the ICAM-1 ELISA. Dr. M. Lappas was a recipient of a National Health and Medical Research Council (NHMRC) RD Wright Fellowship (Grant no. 454777). Funding for the xMark microplate absorbance spectrophotometer was provided by the Medical Research Foundation for Women and Babies.